Wheat TaLr35PR2 gene is required for Lr35-mediated adult plant resistance against leaf rust fungus.
Identifieur interne : 000118 ( Main/Exploration ); précédent : 000117; suivant : 000119Wheat TaLr35PR2 gene is required for Lr35-mediated adult plant resistance against leaf rust fungus.
Auteurs : Fang Liang [République populaire de Chine] ; Xiong Du [République populaire de Chine] ; Jiarui Zhang [République populaire de Chine] ; Xiaoying Li [République populaire de Chine] ; Fei Wang [République populaire de Chine] ; Haiyan Wang [Oman] ; Daqun Liu [République populaire de Chine]Source :
- Functional plant biology : FPB [ 1445-4416 ] ; 2019.
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Abstract
In this study we analysed the expression patterns of TaLr35PR2 and confirmed its role in Lr35-mediated adult resistance to leaf rust fungus. β-1,3-glucanase, a pathogenesis-related protein, has a critical function in plant defence response against fungal pathogens. We previously described the full-length gene TaLr35PR2, which encodes a protein exhibiting amino acid and structural similarity to β-1,3-glucanase, in the wheat near-isogenic line TcLr35 (GenBank accession number DQ294235.1). This work aimed to further assess TaLr35PR2 expression patterns and function in Lr35-mediated adult resistance to Puccinia triticina. Immunoblot was performed to demonstrate that TaLr35PR2 expression was triggered early by P. triticina, with expression levels markedly elevated in incompatible interaction compared with those in compatible one. Additionally, TaLr35PR2 accumulation steadily increased and overtly peaked after challenge with P. triticina through the various developmental stages of TcLr35 wheat, and remaining at similar levels after mock inoculation. Furthermore, TaLr35PR2 expression was significantly reduced in barley stripe mosaic virus (BSMV)-induced gene knockdown plants, in which pathological assessment revealed that TaLr35PR2-silenced plants was obviously susceptible to leaf rust fungus compared with wild-type TcLr35, indicating that Lr35-mediated resistance to leaf rust was diminished. These findings strongly suggest that TaLr35PR2 is involved in Lr35-mediated wheat defence against the leaf rust pathogen.
DOI: 10.1071/FP18340
PubMed: 31813413
Affiliations:
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Email: ndwanghaiyan@163.com.</nlm:affiliation><country wicri:rule="url">Oman</country></affiliation></author><author><name sortKey="Liu, Daqun" sort="Liu, Daqun" uniqKey="Liu D" first="Daqun" last="Liu">Daqun Liu</name><affiliation wicri:level="1"><nlm:affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001</wicri:regionArea><wicri:noRegion>Baoding 071001</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31813413</idno><idno type="pmid">31813413</idno><idno type="doi">10.1071/FP18340</idno><idno type="wicri:Area/Main/Corpus">000104</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000104</idno><idno type="wicri:Area/Main/Curation">000104</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000104</idno><idno type="wicri:Area/Main/Exploration">000104</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Wheat TaLr35PR2 gene is required for Lr35-mediated adult plant resistance against leaf rust fungus.</title><author><name sortKey="Liang, Fang" sort="Liang, Fang" uniqKey="Liang F" first="Fang" last="Liang">Fang Liang</name><affiliation wicri:level="1"><nlm:affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001</wicri:regionArea><wicri:noRegion>Baoding 071001</wicri:noRegion></affiliation></author><author><name sortKey="Du, Xiong" sort="Du, Xiong" uniqKey="Du X" first="Xiong" last="Du">Xiong Du</name><affiliation wicri:level="1"><nlm:affiliation>College of Agronomy, Hebei Agricultural University, Baoding 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agronomy, Hebei Agricultural University, Baoding 071001</wicri:regionArea><wicri:noRegion>Baoding 071001</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Jiarui" sort="Zhang, Jiarui" uniqKey="Zhang J" first="Jiarui" last="Zhang">Jiarui Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001</wicri:regionArea><wicri:noRegion>Baoding 071001</wicri:noRegion></affiliation></author><author><name sortKey="Li, Xiaoying" sort="Li, Xiaoying" uniqKey="Li X" first="Xiaoying" last="Li">Xiaoying Li</name><affiliation wicri:level="1"><nlm:affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001</wicri:regionArea><wicri:noRegion>Baoding 071001</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Fei" sort="Wang, Fei" uniqKey="Wang F" first="Fei" last="Wang">Fei Wang</name><affiliation wicri:level="1"><nlm:affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001</wicri:regionArea><wicri:noRegion>Baoding 071001</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Haiyan" sort="Wang, Haiyan" uniqKey="Wang H" first="Haiyan" last="Wang">Haiyan Wang</name><affiliation wicri:level="1"><nlm:affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China; and Corresponding author. Email: ndwanghaiyan@163.com.</nlm:affiliation><country wicri:rule="url">Oman</country></affiliation></author><author><name sortKey="Liu, Daqun" sort="Liu, Daqun" uniqKey="Liu D" first="Daqun" last="Liu">Daqun Liu</name><affiliation wicri:level="1"><nlm:affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001</wicri:regionArea><wicri:noRegion>Baoding 071001</wicri:noRegion></affiliation></author></analytic><series><title level="j">Functional plant biology : FPB</title><idno type="eISSN">1445-4416</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (MeSH)</term><term>Gene Knockdown Techniques (MeSH)</term><term>Plant Diseases (MeSH)</term><term>Triticum (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (MeSH)</term><term>Maladies des plantes (MeSH)</term><term>Techniques de knock-down de gènes (MeSH)</term><term>Triticum (MeSH)</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Basidiomycota</term><term>Gene Knockdown Techniques</term><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Basidiomycota</term><term>Maladies des plantes</term><term>Techniques de knock-down de gènes</term><term>Triticum</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study we analysed the expression patterns of TaLr35PR2 and confirmed its role in Lr35-mediated adult resistance to leaf rust fungus. β-1,3-glucanase, a pathogenesis-related protein, has a critical function in plant defence response against fungal pathogens. We previously described the full-length gene TaLr35PR2, which encodes a protein exhibiting amino acid and structural similarity to β-1,3-glucanase, in the wheat near-isogenic line TcLr35 (GenBank accession number DQ294235.1). This work aimed to further assess TaLr35PR2 expression patterns and function in Lr35-mediated adult resistance to Puccinia triticina. Immunoblot was performed to demonstrate that TaLr35PR2 expression was triggered early by P. triticina, with expression levels markedly elevated in incompatible interaction compared with those in compatible one. Additionally, TaLr35PR2 accumulation steadily increased and overtly peaked after challenge with P. triticina through the various developmental stages of TcLr35 wheat, and remaining at similar levels after mock inoculation. Furthermore, TaLr35PR2 expression was significantly reduced in barley stripe mosaic virus (BSMV)-induced gene knockdown plants, in which pathological assessment revealed that TaLr35PR2-silenced plants was obviously susceptible to leaf rust fungus compared with wild-type TcLr35, indicating that Lr35-mediated resistance to leaf rust was diminished. These findings strongly suggest that TaLr35PR2 is involved in Lr35-mediated wheat defence against the leaf rust pathogen.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">31813413</PMID><DateCompleted><Year>2020</Year><Month>06</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>06</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1445-4416</ISSN><JournalIssue CitedMedium="Internet"><Volume>47</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>01</Month></PubDate></JournalIssue><Title>Functional plant biology : FPB</Title><ISOAbbreviation>Funct Plant Biol</ISOAbbreviation></Journal><ArticleTitle>Wheat TaLr35PR2 gene is required for Lr35-mediated adult plant resistance against leaf rust fungus.</ArticleTitle><Pagination><MedlinePgn>26-37</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1071/FP18340</ELocationID><Abstract><AbstractText>In this study we analysed the expression patterns of TaLr35PR2 and confirmed its role in Lr35-mediated adult resistance to leaf rust fungus. β-1,3-glucanase, a pathogenesis-related protein, has a critical function in plant defence response against fungal pathogens. We previously described the full-length gene TaLr35PR2, which encodes a protein exhibiting amino acid and structural similarity to β-1,3-glucanase, in the wheat near-isogenic line TcLr35 (GenBank accession number DQ294235.1). This work aimed to further assess TaLr35PR2 expression patterns and function in Lr35-mediated adult resistance to Puccinia triticina. Immunoblot was performed to demonstrate that TaLr35PR2 expression was triggered early by P. triticina, with expression levels markedly elevated in incompatible interaction compared with those in compatible one. Additionally, TaLr35PR2 accumulation steadily increased and overtly peaked after challenge with P. triticina through the various developmental stages of TcLr35 wheat, and remaining at similar levels after mock inoculation. Furthermore, TaLr35PR2 expression was significantly reduced in barley stripe mosaic virus (BSMV)-induced gene knockdown plants, in which pathological assessment revealed that TaLr35PR2-silenced plants was obviously susceptible to leaf rust fungus compared with wild-type TcLr35, indicating that Lr35-mediated resistance to leaf rust was diminished. These findings strongly suggest that TaLr35PR2 is involved in Lr35-mediated wheat defence against the leaf rust pathogen.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Fang</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Xiong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Agronomy, Hebei Agricultural University, Baoding 071001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jiarui</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xiaoying</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Fei</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Haiyan</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China; and Corresponding author. Email: ndwanghaiyan@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Daqun</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Centre of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Australia</Country><MedlineTA>Funct Plant Biol</MedlineTA><NlmUniqueID>101154361</NlmUniqueID><ISSNLinking>1445-4416</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="Y">Basidiomycota</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055785" MajorTopicYN="N">Gene Knockdown Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="Y">Triticum</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>10</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>07</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>12</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>6</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>12</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31813413</ArticleId><ArticleId IdType="pii">FP18340</ArticleId><ArticleId IdType="doi">10.1071/FP18340</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Oman</li><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Liang, Fang" sort="Liang, Fang" uniqKey="Liang F" first="Fang" last="Liang">Fang Liang</name></noRegion><name sortKey="Du, Xiong" sort="Du, Xiong" uniqKey="Du X" first="Xiong" last="Du">Xiong Du</name><name sortKey="Li, Xiaoying" sort="Li, Xiaoying" uniqKey="Li X" first="Xiaoying" last="Li">Xiaoying Li</name><name sortKey="Liu, Daqun" sort="Liu, Daqun" uniqKey="Liu D" first="Daqun" last="Liu">Daqun Liu</name><name sortKey="Wang, Fei" sort="Wang, Fei" uniqKey="Wang F" first="Fei" last="Wang">Fei Wang</name><name sortKey="Zhang, Jiarui" sort="Zhang, Jiarui" uniqKey="Zhang J" first="Jiarui" last="Zhang">Jiarui Zhang</name></country><country name="Oman"><noRegion><name sortKey="Wang, Haiyan" sort="Wang, Haiyan" uniqKey="Wang H" first="Haiyan" last="Wang">Haiyan Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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